Dissociative mechanism for irreversible thermal denaturation of oligomeric proteins
نویسندگان
چکیده
منابع مشابه
Thermal Denaturation Equilibria of Tmv Coat Proteins
Thermal denaturation of RNA free coat proteins of tobacco mosaic virus (TMV) was studied for wildtype TMV (vulgare) and the temperature-sensitive mutant, Ni 118. The ability to form soluble aggregates as well as the optical properties (ORD, UV-difference spectra), and the sedimen tation behavior were used as criteria for the native state. At pH 7.5 , 7 = 0 .0 2 denaturation is reversible for b...
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We study cold denaturation of proteins at high pressures. Using multicanonical Monte Carlo simulations of a model protein in a water bath, we investigate the effect of water density fluctuations on protein stability. We find that above the pressure where water freezes to the dense ice phase (approximately 2 kbars) the mechanism for cold denaturation with decreasing temperature is the loss of lo...
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ژورنال
عنوان ژورنال: Biophysical Reviews
سال: 2016
ISSN: 1867-2450,1867-2469
DOI: 10.1007/s12551-016-0220-z